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1 final ph should be 7.6 ± 0.2 at 25°c Subsequent subculturing may be carried out in liquid medium or on agar plates. If using the complete medium from difco add 37.40 g to 1 litre water
M.514 refers to bacto marine broth difco 2216 (bd 279110) 1 rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid medium 514 Other brands (even if they refer to difco2216) have not been tested and might not support growth for some strains.
If using difco bacto marine broth use 18.7 g/l to 1 litre water
2 the medium may be solidified by adding 15 g/l agar. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% n 2 gas Medium for oceanimonas smirnovii dsm 108803 this strain uses the standard medium without modifications. We recommend using the medium supplied by carl roth gmbh + co
Kg for marinomonas mediterranea dsm 23531. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% n2 gas 1 use medium 514 to which is added nacl at the concentration indicated (i.e 514 + 6% nacl indicates use medium 514 to which 60 g/l nacl has been added
Modified bacto marine broth this medium was extracted automatically and has not been curated by experts, yet.
Discover standardized cultivation media recipes for more than 40,000 microbial strains.
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