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Glori is an unbiased, convenient method for the absolute quantification of the m6a methylome For glori, a's are converted into g's We apply glori to quantify the m 6 a methylomes of mouse and human cells and reveal clustered m 6 a modifications with differential distribution and stoichiometry.
Recently, we developed glori, a sequencing method … more Make sure that you have reads which match the real strandness of rna The process seamlessly combines chemical precision, sequencing, and bioinformatics for reliable m6a profiling, advancing your research in rna regulation.
However, its lengthy reaction time and severe rna degradation have limited its.
This work introduces glori 2.0 and 3.0, enabling sensitive m6a quantification from rna inputs as low as a few hundred cells.
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